Cullin 4a polyclonal, anti-human

Background:
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition.

Alternative Name:
CUL-4A, CUL4A, 2810470J21Rik, AW495282, CUL 4A, CUL-4A, Cul4a, Cul4a protein, CUL4A, Cullin-4A, MGC36573, MGC64071,

Application Dilution:
WB:1:500-2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: Cullin 4a polyclonal antibody detects endogenous levels of Cullin 4a protein.

Immunogen:
Recombinant protein

MW: 88 kDa

Swiss-Prot: Q13619(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.