CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)

Detection Principle:
Superoxide anion (O2•-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2•-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.
Instrument: Microplate reader
Assay Time: 70 min

Tested Sample Types: Serum,plasma,urine,cells,cell culture supernatant,tissue

Research Area's: Enzymes,Oxidative stress

Shelf Life: 6 Months

Storage:
Reagent4:-20℃;Others:2-8℃

Citations:


Detection Principle: http://nopr.niscair.res.in/handle/123456789/44593
https://www.researchgate.net/profile/Yahya_Shekha2/publication/337951595
https://www.researchgate.net/profile/Abdulkareem_Jewad/publication/337783411
https://jbiochemtech.com/storage/models/article/KnsVSU1NXsBNqxGiJnGD5yDFyz5DuySl83dgLkBwCoqOrXsCgr5x0nXHQ4Mf/
https://pubmed.ncbi.nlm.nih.gov/32768965/
https://pubmed.ncbi.nlm.nih.gov/32767829/
https://pubmed.ncbi.nlm.nih.gov/32437678/
https://pubmed.ncbi.nlm.nih.gov/32724495/
https://pubmed.ncbi.nlm.nih.gov/32848317/