Cyclin B2 polyclonal, anti-human, mouse, rat

Background:
In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of control of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex . Evidence has established that B-type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B-type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.

Alternative Name:
CCNB2, ccnb2, CCNB2, CycB2, Cyclin B2, G2 mitotic specific cyclin B2, G2/mitotic specific cyclin B2, G2/mitotic-specific cyclin-B2, HsT17299, MGC108931, MGC140694,

Application Dilution:
WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Specificity: Cyclin B2 polyclonal antibody detects endogenous levels of Cyclin B2 protein.

Immunogen:
recombinant protein

MW: 45 kDa

Swiss-Prot: O95067(Human) P30276(Mouse) EntrezGene:363088(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.