Cyclin E1 (phospho-T395) polyclonal, anti-human, mouse, rat
€328.00
In stock
SKU
BS4057
Background:
Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner.
Alternative Name:
G1/S-specific cyclin-E1, CCNE1, CCNE
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200
Specificity: p-Cyclin E1 (T395) polyclonal antibody detects endogenous levels of Cyclin E1 protein only when phosphorylated at Thr395
Immunogen:
Synthetic phosphopeptide derived from human Cyclin E1 around the phosphorylation site of Threonine 395.
MW: ~ 48 kDa
Swis Prot.: P24864
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner.
Alternative Name:
G1/S-specific cyclin-E1, CCNE1, CCNE
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200
Specificity: p-Cyclin E1 (T395) polyclonal antibody detects endogenous levels of Cyclin E1 protein only when phosphorylated at Thr395
Immunogen:
Synthetic phosphopeptide derived from human Cyclin E1 around the phosphorylation site of Threonine 395.
MW: ~ 48 kDa
Swis Prot.: P24864
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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