Cyclin H polyclonal, anti-human, mouse

Background:
Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit, Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires, in addition, phosphorylation at Thr-160. The enzyme responsible for phosphorylation of Thr-160 in Cdk2 and also Thr-161 in Cdc2 p34, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit and a regulatory subunit. The catalytic subunit, designated Cdk7, has been identified as the mammalian homolog of MO15, a protein kinase demonstrated earlier in starfish and Xenopus. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. Like other Cdks, Cdk7 contains a conserved threonine required for full activity, mutation of this residue severely reduces CAK activity.

Alternative Name:
CCNH, 6330408H09Rik, AI661354, AV102684, AW538719, CAK, CAK complex subunit, ccnh, CCNH, CDK activating kinase, CDK activating kinase complex subunit, Cyclin dependent kinase activating kinase, cyclin dependent kinase activating kinase complex subunit, Cyclin H, Cyclin-H, CyclinH, MO15 associated protein, MO15-associated protein, p34, p36, p37,

Application Dilution:
WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Specificity: Cyclin H polyclonal antibody detects endogenous levels of Cyclin H protein.

Immunogen:
recombinant protein

MW: 38 kDa

Swiss-Prot: P51946(Human) Q61458(Mouse)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.