CYCS Polyclonal Antibody

Background:
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation,cytochrome c can be released from mitochondria into cytoplasm,resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition,cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.

Research Area: Cancer, Cell Biology, Cardiovascular, Metabolism, Signal Transduction

Synonyms:
Cytochrome C, Somatic, CYC, Cytochrome C, THC4, HCS.

Immunogen: Recombinant Human Cytochrome c protein

Swissprot: P99999

Calculated MW: 12 kDa
Observed MW: 12 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.

Concentration: 2 mg/mL
Dilution: WB 1:1000-1:2000 IHC 1:100-1:200

Storage:
Store at -20°C. Avoid freeze / thaw cycles.