CYCS Polyclonal Antibody

Background:
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.

Research Area: Cancer, Cell Biology, Cardiovascular, Metabolism, Signal Transduction

Synonyms:
Cytochrome C, Somatic, CYC, Cytochrome C, THC4, HCS.

Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse Cytochrome c

Swissprot: P99999,P62898,P62897

Calculated MW: 12 kDa
Observed MW: 12 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4

Concentration: 1.5 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:500-1:1000, IF 1:200-1:800

Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Citation(s):
https://link.springer.com/article/10.1007/s11696-019-00894-8