Cylicin I polyclonal, anti-human, bovine

Quantity: 25 µl

Background:
This gene encodes a sperm head cytoskeletal protein. The encoded protein is associated with the calyx of spermatozoa and spermatids. Alternative splicing results in multiple transcript variants.

Synonyms:
CYCL1

Isotype: Guinea Pig Polyclonal (Cyl I-1)

Immunogen:
Human recombinant cylicin I, coupled to KLH

Form: Stabilized Serum

Specificity:
Cylicin I (MW 74,788, deduced from sequence analysis), a basic protein (IEP 10.55) of the sperm head cytoskeleton with Mr 74,000 on SDS-PAGE; multiple band proteins with MW 58, 63, 69 and 74 kDa of calyx-associated polypeptides of bovine spermatozoa. Especially useful in discrimination of morphologically altered round-headed spermatozoa.

Reactivity: human, bovine

Applications: Immunohistochemistry on frozen sections, Immunoblotting (western)

Working DIlution: 1:100 for immunohistochemistry 1:2,000 for Western blotting (ECL)

Storage: 2-8C

References:
- Hess, H..et al. (1993) J. Cell Biol 5: 1043-1052
- Hess, H..et al. (1995) Exp Cell Res 218: 174-182