Cylicin II polyclonal, anti-human, bovine

Quantity: 25 µl

Background:
Cylicin II (CYCL2) is specifically expressed in testis and is part of the cytoskeletal calyx of mammalian sperm heads. Cylicin II may play a role in the morphogenesis of the sperm head.

Synonyms:
CYCL2

Isotype: Guinea Pig Polyclonal (Cyl II-2)

Immunogen:
Bovine recombinant cylicin II, coupled to KLH

Form: Stabilized Serum

Specificity:
Cylicin II (MW 53,561, deduced from sequence analysis), a basic protein (IEP 10.55) of the sperm head cytoskeleton with Mr 58,000 on SDS-PAGE; multiple band proteins with MW 58, 63, 69 and 74 kDa of calyx-associated polypeptides of bovine spermatozoa. Especially useful in discrimination of morphologically altered round-headed spermatozoa

Reactivity: human, bovine

Applications: Immunohistochemistry on frozen sections, Immunoblotting (western)

Working DIlution: 1:100 for immunohistochemistry 1:2,000 for Western blotting (ECL)

Storage: 2-8C

References:
- Hess, H..et al. (1993) J. Cell Biol 5: 1043-1052
- Hess, H..et al. (1995) Exp Cell Res 218: 174-182"