CYP11A1 polyclonal, anti-mouse, rat

Quantity: 100 µg

Background:
CYP11A1(Cytochrome p450, family 11, subfamily A, polypeptide 1), also called CYTOCHROME P450SCC, CYTOCHROME P450C11A1 or CYP11A, is a mitochondrial enzyme associated with the conversion of cholesterol to pregnenolone. CYP11A1 is a member of the cytochrome P450 superfamily of enzymes. The CYP11A1 gene is mapped on 15q24.1. Expression of the CYP11A1 gene may play a role in skin physiology and pathology and that cutaneous proopiomelanocortin activity may be autoregulated by a feedback mechanism involving glucocorticoids synthesized locally by Slominski et al. Using in vitro studies, CYP11A1 catalyzed the side-chain cleavage of 7-dehydrocholesterol to form 7-dehydropregnenolone. In addition, CYP11A1 catalyzed the metabolism of biologically inert vitamin D3, which is formed from 7-dehydrocholesterol, to form 2 hydroxylated products, 20-hydroxyvitamin D3 and 20, 22-dihydroxyvitamin D3. Mutations in the CYP11A1 gene cause congenital adrenal insufficiency with partial or complete 46, XY sex reversal.

Description:
Rabbit IgG polyclonal antibody for Cholesterol side-chain cleavage enzyme, mitochondrial (CYP11A1) detection. Tested with WB in Mouse, Rat.

Synonyms:
Cholesterol 20 22 desmolase antibody, Cholesterol desmolase antibody, Cholesterol monooxygenase(side chain cleaving) antibody, Cholesterol side chain cleavage enzyme antibody, Cholesterol side chain cleavage enzyme mitochondrial antibody, Cholesterol side-chain cleavage enzyme antibody, CP11A_HUMAN antibody, CYP 11A antibody, CYP11A antibody, CYP11A1 antibody, CYPXIA1 antibody, Cytochrome P450 11A1 antibody, Cytochrome P450 11A1 mitochondrial antibody, Cytochrome P450 family 11 subfamily A polypeptide 1 antibody, Cytochrome P450 subfamily XIA(cholesterol side chain cleavage) antibody, Cytochrome P450 subfamily XIA antibody, Cytochrome P450(scc) antibody, Cytochrome P450C11A1 antibody, mitochondrial antibody, P450(scc) antibody, P450SCC antibody, Steroid 20 22 lyase antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of mouse CYP11A1(53-66aa DNGWLNLYHFWRES), different from the related rat sequence by two amino acids.A synthetic peptide corresponding to a sequence at the N-terminus of mouse CYP11A1(53-66aa DNGWLNLYHFWRES), different from the related rat sequence by two amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: mouse, rat

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.