CYP1A1 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
CYP1A1 is involved in phase I xenobiotic and drug metabolism (one substrate of it is theophylline). It is inhibited by fluoroquinolones and macrolides and induced by aromatic hydrocarbons. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH), for example, benzo(a)pyrene (BP), by transforming it to an epoxide. In this reaction, the oxidation of benzo[a]pyrene is catalysed by CYP1A1 to form BP-7,8-epoxide, which can be further oxidized by epoxide hydrolase (EH) to form BP-7,8-dihydrodiol. Finally CYP1A1 catalyses this intermediate to form BP-7,8-dihydrodiol-9,10-epoxide, which is the ultimate carcinogen. However, an in vivo experiment with gene-deficient mice has found that the hydroxylation of benzo(a)pyrene by CYP1A1 can have an overall protective effect on the DNA, rather than contributing to potentially carcinogenic DNA modifications. This effect is likely due to the fact that CYP1A1 is highly active in the intestinal mucosa, and thus inhibits infiltration of ingested benzo(a)pyrene carcinogen into the systemic circulation.

Description:
Rabbit IgG polyclonal antibody for Cytochrome P450 1A1 (CYP1A1) detection. Tested with WB, IHC-P, IHC-F in Human, Mouse, Rat.

Synonyms:
AHH antibody, AHRR antibody, Aryl hydrocarbon hydroxylase antibody, CP 11 antibody, CP11 antibody, CP1A1_HUMAN antibody, CYP 1 antibody, CYP1 antibody, CYP1A1 antibody, CYPIA1 antibody, Cytochrome P1 450 dioxin inducible antibody, Cytochrome P1-450 antibody, Cytochrome P1-450 dioxin-inducible antibody, Cytochrome P450 1A1 antibody, Cytochrome P450 family 1 subfamily A polypeptide 1 antibody, Cytochrome P450 form 6 antibody, Cytochrome P450 subfamily I (aromatic compound inducible) polypeptide 1 antibody, Cytochrome P450 subfamily I aromatic compound inducible polypeptide 1 antibody, Cytochrome P450-C antibody, Cytochrome P450-P1 antibody, Flavoprotein-linked monooxygenase antibody, Microsomal monooxygenase antibody, P1 450 antibody, P450 C antibody, P450 form 6 antibody, P450 P1 antibody, P450DX antibody, Xenobiotic monooxygenase antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
E.coli-derived human CYP1A1 recombinant protein (Position: H183-D320). Human CYP1A1 shares 81.2% amino acid (aa) sequence identity with both mouse and rat CYP1A1.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P, IHC-F

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.