CYP7A1 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
CYP7A1 (Cytochrome P450 Subfamily VIIA Polypeptide 1), also called CYP7 or CHOLESTEROL 7-ALPHA -HYDROXYLASE, is an enzyme that in humans is encoded by the CYP7A1 gene. Using both mouse-human somatic cell hybrids and in situ chromosomal hybridization, Cohen et al. (1992) mapped the CYP7 gene to 8q11-q12. By transfection of reporter constructs, mutation analysis, and DNase footprinting, Molowa et al. (1992) identified areas of the CYP7A1 promoter region that showed hepatocyte-specific activation. They found HNF3 to be an activator of CYP7A1 activity. Agellon et al. (2002) found that wildtype mice and mice transgenic for human CYP7A1 respond differently to cholesterol feeding. Cholesterol feeding stimulated Cyp7a1 mRNA abundance and enzymatic activity in wildtype mice, but repressed human CYP7A1 mRNA and activity in transgenic mice.

Description:
Rabbit IgG polyclonal antibody for Cholesterol 7-alpha-monooxygenase (CYP7A1) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
Cholesterol 7 alpha hydroxylase antibody, Cholesterol 7 alpha monooxygenase antibody, cholesterol 7 alpha-monooxygenase antibody, Cholesterol 7-alpha-hydroxylase antibody, Cholesterol 7-alpha-monooxygenase antibody, CP7A antibody, CP7A1_HUMAN antibody, CYP 7 antibody, CYP7 antibody, CYP7A1 antibody, CYPVII antibody, Cytochrome P450 7A1 antibody, cytochrome P450, family 7, subfamily A, polypeptide 1 antibody, Cytochrome P450, subfamily VIIA (cholesterol 7 alpha monooxygenase), antibody, cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), antibody, Cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), polypeptide 1 antibody, MGC126826 antibody, MGC138389 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
E.coli-derived human CYP7A1 recombinant protein (Position: M301-L504). Human CYP7A1 shares 85.2 % and 83.3% amino acid (aa) sequence identity with mouse and rat CYP7A1, respectively.E.coli-derived human CYP7A1 recombinant protein (Position: M301-L504). Human CYP7A1 shares 85.2 % and 83.3% amino acid (aa) sequence identity with mouse and rat CYP7A1, respectively.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: mouse, rat.
Predicted to work with: human.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.