Cytochrome C polyclonal, anti-human, mouse, rat

Background:
Cytochrome c is a well characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial intermembrane space. More recent studies have identifed cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is translocated from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

Alternative Name:
CYCS, CYC, CYC, CYCS, Cytochrome c, Cytochrome c somatic, HCS, THC4,

Application Dilution:
WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Specificity: Cytochrome C polyclonal antibody detects endogenous levels of Cytochrome C protein.

Immunogen:
recombinant protein

MW: 14 kDa

Swiss-Prot: P99999(Human) P62897(Mouse) P62898(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.