Cytochrome P450 2E1 polyclonal, anti-mouse, rat

Quantity: 100 µg

Background:
CYP2E1, also known as P450IIE1, is a member of the P450IIE subfamily which is ethanol-inducible. It has at least 1 gene which is mapped to 10q24.3-qter, and a second is likely in rat and in man. Both the rat and human proteins encoded by this gene contain 493 amino acids and calculated molecular masses of 56,634 and 56,916 daltons, respectively. In addition, genetic polymorphisms in the 5-prime flanking region of the human P450IIE1 gene affected its binding of transacting factor and changed its transcriptional regulation, which may lead to interindividual differences of microsomal drug oxidation activity. P450IIE1 is an important enzyme for the catalysis of the conversion of ethanol to acetaldehyde and to acetate in humans,and it is also involved in the metabolism of nitrosamines. Due to the possible correlation of P450IIE1 genes with malignancy, clinical studies of RFLP patterns of these genes in cancer may be useful.

Description:
Rabbit IgG polyclonal antibody for Cytochrome P450 2E1 (CYP2E1) detection. Tested with WB, IHC-P in Mouse, Rat.

Synonyms:
4-nitrophenol 2-hydroxylase antibody, CP2E1_HUMAN antibody, CPE 1 antibody, CPE1 antibody, CYP2E antibody, CYP2E1 antibody, CYPIIE1 antibody, Cytochrome P450 2E1 antibody, Cytochrome P450 family 2 subfamily E polypeptide 1 antibody, Cytochrome P450 isozyme 3A antibody, Cytochrome P450 LM3A antibody, Cytochrome P450 subfamily IIE(ethanol inducible) polypeptide 1 antibody, Cytochrome P450 subfamily IIE polypeptide 1 antibody, Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody, cytochrome P450, subfamily IIE(ethanol-inducible), polypeptide 1 antibody, Cytochrome P450, subfamily IIE antibody, Cytochrome P450-ALC antibody, Cytochrome P450-J antibody, Cytochrome P450RLM6 antibody, EC 1.14.13.- antibody, EC 1.14.13.n7 antibody, Ethanol-inducible P450 antibody, Flavoprotein linked monooxygenase antibody, Flavoprotein-linked monooxygenase antibody, MGC133529 antibody, Microsomal monooxygenase antibody, OTTHUMP00000020813 antibody, OTTHUMP00000046571 antibody, P450 ALC antibody, p450 antibody, P450 J antibody, P450-J antibody, P450C2E antibody, P450J antibody, P450RLM6 antibody, Xenobiotic monooxygenase antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of mouse Cytochrome P450 2E1(94-112aa KNEFSGRGDIPVFQEYKNK), identical to the related rat sequence.A synthetic peptide corresponding to a sequence at the N-terminus of mouse Cytochrome P450 2E1(94-112aa KNEFSGRGDIPVFQEYKNK), identical to the related rat sequence.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: mouse, rat

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.