Cytokeratin 10+13 (Keratin K10+K13) (clone )DE-K13, anti-human, feline, zebrafish

Background:
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Clone: DE-K13

Isotype: Mouse IgG2a

Source: DE-K13 is a Mouse monoclonal IgG2a, k antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a (BALB/c x B6)F1 Mouse immunized with a cytoskeletal prepaRation extracted from Human ectocervical epithelium.

Reactivity: feline, human, zebrafish

Specificity: DE-K13 reacts with Cytokeratin 10 and 13 in fixed cells and on frozen tissues, but exclusively with Cytokeratin 13 in formalin-fixed, paraffin-embedded tissues. Cytokeratin 10 is present in Keratinizing stRatified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas, while Cytokeratin 13 is present in non-cornified squamous epithelia, except cornea, and transitional epithelial regions, with the exception of basal cell layers of some stRatified epithelia, as well as carcinomas derived from these tissues.

Form: Each vial contains 100ul purified monoclonal antibody in PBS containing 0.09% sodium azide.

Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Western blotting

Storage: Store at 4C, or in small aliquots at -20C.