Cytokeratin 16 (Keratin K16) (clone LL025), anti-human

Background:
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Clone: LL025

Isotype: Mouse IgG1

Source: LL025 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 murine myeloma cells with spleen cells from a Mouse immunized with a synthetic peptide corresponding to an amino acid sequence of the carboxyl terminus of Human Keratin 16.

Reactivity: human

Specificity: Keratin 16 is specifically found in hyperprolifeRative squamous epithelium such as psoriatic lesions and squamous cell carcinomas, e.g. of the lung. It is expressed in suprabasal Keratinocytes of wounded epidermis, a situation of high cell turnover. LL025 reactivity is negative in, e.g. normal skin, breast and lung tissue and non-invasive breast carcinoma. However, 10% of the invasive breast carcinomas tested were diffusely or focally positive with this antibody.

Form: Each vial contains 100ul purified monoclonal antibody in PBS containing 0.09% sodium azide.

Applications: Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Western blotting

Storage: Store at 4C, or in small aliquots at -20C.