Cytokeratin 18 (phospho-S52) polyclonal, anti-human

Background:
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments. Keratin isoforms demonstrate tissue and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases.

Alternative Name:
Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18, CYK18, PIG46

Application Dilution:
WB: 1:500~1:1000

Specificity: p-Cytokeratin 18 (S52) polyclonal antibody detects endogenous levels of Cytokeratin 18 protein only when phosphorylated at Ser52.

Immunogen:
Synthetic phosphopeptide derived from human Cytokeratin 18 around the phosphorylation site of Serine 52

MW: ~ 46 kDa

Swiss-Prot: P05783

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.