Cytokeratin 18 polyclonal, anti-human, mouse, rat

Background:
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, Cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while Cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas. Cytokeratin 18 contains two major phosphorylation sites on Ser 33 and Ser 52. Phosphorylation of Ser 18 is essential for the association of Cytokeratin 18 with 14-3-3 proteins and is involved in keratin organization and distribution.

Alternative Name:
CK-18, K18, KRT18, Cell proliferation inducing gene 46 protein, Cell proliferation inducing protein 46, Cell proliferation-inducing gene 46 protein, CK 18, CK-18, CK18, CYK 18, CYK18, Cytokeratin 18, Cytokeratin endo B, Cytokeratin-18, K 18, K18, K1C18, KA18, Keratin 18, Keratin 18, type I, Keratin D, keratin, type I cytoskeletal 18, Keratin-18, Krt18,

Application Dilution:
WB:1:10,000-1:50,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Specificity: Cytokeratin 18 polyclonal antibody detects endogenous levels of Cytokeratin 18 protein.

Immunogen:
recombinant protein

MW: 48 kDa

Swiss-Prot: P05783(Human) P05784(Mouse) Q5BJY9(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.