Cytokeratin 4 (Keratin K4) (clone 6B10), anti-human, canine, feline, zebrafish

Background:
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Clone: 6B10

Isotype: Mouse IgG1

Source: 6B10 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a Cytokeratin prepaRation extracted from Human esophagus.

Reactivity: canine, feline, human, zebrafish

Specificity: 6B10 reacts exlcusively with Cytokeratin 4 which is present in non-cornifying squamous epithelium, including cornea and transitional epithelium. Cells in certain ciliated pseudo-stRatified epithelia and ductal epithelia of various exocrine glands are also positive for 6B10.

Form: Each vial contains 100ul purified monoclonal antibody in PBS containing 0.09% sodium azide.

Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western blotting

Storage: Store at 4C, or in small aliquots at -20C.