Cytokeratin 7 (KRT7) (Glandular and Transitional Epithelial Marker) (clone KRT7/760 + KRT7/903), anti-human

Cytokeratin 7 (KRT7) (Glandular and Transitional Epithelial Marker) (clone KRT7/760 + KRT7/903), anti-human

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In stock
SKU
ARP-24-3855-MSM5
Catalog Number: 24-3855-MSM5
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG's
Decription:
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This monoclonal antibody is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This monoclonal antibody is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Synonyms: CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

Clone: KRT7/760 + KRT7/903

Isotype: Mouse IgG's

Immunogen: Recombinant full-length human KRT7 protein

Reactivity: human. Others not known.

Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Reactivity: HeLa cells. Carcinoma of ovary, lung, cervix, or breast

Concentration: 0.2mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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