Cytokeratin 8 (phospho-S74) polyclonal, anti-human, mouse, rat

Cytokeratin 8 (phospho-S74) polyclonal, anti-human, mouse, rat

€328.00
In stock
SKU
BS4677
Catalog Number: BS4677
Size: 50 ul, 100 ul
Isotype: rabbit IgG
Applications: WB, IHC

Datasheet
Background:
Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.

Alternative Name:
Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, Short name=K8, Type-II keratin Kb8, KRT8, CYK8

Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200

Specificity: p-Cytokeratin 8 (S74) polyclonal antibody detects endogenous levels of Cytokeratin 8 protein only when phosphorylated at Ser74.

Immunogen:
Synthetic phosphopeptide derived from human Cytokeratin 8 around the phosphorylation site of Serine 74.

MW: ~ 55 kDa

Swis Prot.: P05787

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.
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