Cytokeratin Basic (Type II or HMW) (Epithelial Marker) (clone KRTH/1076), anti-human, rat

Cytokeratin Basic (Type II or HMW) (Epithelial Marker) (clone KRTH/1076), anti-human, rat

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In stock
SKU
ARP-24-MSM9-1076
Catalog Number: 24-MSM9-1076
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG1, kappa
Decription:
This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Synonyms: KRT2B, KRT2P, HUMCYT2A, Keratin, Type II Cytoskeletal 2 Oral, K2P, K76, CK-2P, Keratin 2p, Keratin-76, Cytokeratin 2, Cytokeratin-2P, Type-II Keratin Kb9

Clone: KRTH/1076

Isotype: Mouse IgG1, kappa

Immunogen: Recombinant human KRT76 protein

Reactivity: human and rat. Shows broad species reactivity.

Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Applications: ELISA (For coating, order Ab without BSA)Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blotting (0.5-1ug/ml f)
Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Reactivity: Skin, Adenocarcinomas

Concentration: 0.2mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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