Cytokeratin Basic (Type II or HMW) (Epithelial Marker) (clone SPM591), anti-human, mouse, rat

Cytokeratin Basic (Type II or HMW) (Epithelial Marker) (clone SPM591), anti-human, mouse, rat

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In stock
SKU
ARP-24-MSM3X-446
Catalog Number: 24-MSM3X-446
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG1, kappa
Decription:
This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This monoclonal antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.

Clone: SPM591

Isotype: Mouse IgG1, kappa

Immunogen: Solubilized keratin extract from human stratum corneumUnigeneMultiple

Reactivity: human, mouse, rat. Others not known.

Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1.0ug/ml)
Western Blotting (0.5-1.0ug/ml)
Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Reactivity: PC12 cells, Skin, Prostate carcinoma

Concentration: 0.2mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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