Cytokeratin/Keratin 1 (Keratin K1) polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Synonyms:
KRT1, K1, CK1, EHK, EHK1, EPPK, KRT1A, NEPPK

Isotype: Guinea pig Polyclonal (K1.1)

Immunogen:
Synthetic peptide (SAGIINYQRRTTSS-C) of human cytokeratin 1 conjugated to KLH

Form: Stabilized Serum

Specificity:
Basic human keratin K1 (Mr 66 000). Specific for keratin K1 in suprabasal layers of cornified squamous epithelia, e.g. the suprabasal layer of scalp and foot sole. Negative with non-keratinizing stratified and non-stratified epithelia (e.g. esophagus, duodenum).

Reactivity: human, mouse

Applications: Immunohistochemistry on frozen and paraffin sections (after microwave treatment or trypsin), Immunoblotting (western, ECL), Cytological material

Working DIlution: 1:200 for frozen sections; 1:100 for paraffin sections; 1:3000 for Immunoblotting

Positive Control:
Human foot sole

Storage: 2-8C

References:
- Moll R. et al. (1982) Cell 31: 11-24