Cytokeratin/Keratin 15 polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2.

Synonyms:
KRT15, K15, CK15, K1CO

Isotype: Guinea pig Polyclonal (gp 15.1)

Immunogen:
C-terminal tail region (including parts of the rod domain) of human recombinant keratin K15

Form: Stabilized Serum

Specificity:
Mr 50 kD (pI 4.9) intermediate filament polypeptide, keratin K15 (formerly also designated cytokeratin 15), detected by immunofluorescence microscopy in basal cells of stratified epithelia (including epidermis, tongue, esophagus, exocervix) and complex stratified epithelia (e.g. broncheal epithelium), in luminal epithelia of mammary, salivary and sweat gland. In the hair follicle, staining was restricted to the outermost cell layer of the outer root sheath; within the hair follicle several cell layers of the hair follicle bulb were positive with antiserum gp 15.1. In human fetal tissues keratin K15 was localized in basal cells of epidermis, lung, trachea, tracheal glands and esophagus. In fetal hair anlagen, all cells were positive with antiserum gp 15.1, suggesting that keratin K15 is preferentially expressed in epithelial stem cells. Completely unreactive were cells of liver, colon, kidney, urinary bladder, pancreas and lung alveolus. In tumors, gp15.1 is reactive with squamous skin and lung carcinomas and with a significant subgroup of invasive ductal breast carcinomas, especially tumors with higher grade malignancy (Heid, Bartek, Leube, Moll, Kaufmann & Franke, unpublished results).

Reactivity: human, mouse

Applications: Immunohistochemistry on frozen sections, Immunoblotting

Working DIlution: 1:100 to 1:200 for immunohistochemistry; 1:2,000 for immunoblotting (ECL method)

Positive Control:
HaCat, A-431; negative on PLC

Storage: 2-8C

References:
- Jih, DM. et al. (1999) J. Cutan. Pathol. 26: 113-118
- Heid, HW. Et al. (manuscript in preparation)Boehnke K et al. (2007) Eur J Cell Biol 86: 731-746