Cytokeratin/Keratin 2e polyclonal, anti-human, bovine, mouse

Quantity: 25 µl

Background:
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues.

Synonyms:
K2e, KRTE, CK-2e, KRT2A, KRT2E

Isotype: Guinea pig Polyclonal (GP HK2e-Am2)

Immunogen:
Synthetic peptide (N-terminal aa 2-23) of human keratin K2

Form: Stabilized Serum

Specificity:
Basic human keratin K2 (MW 65,852; formerly also designated cytokeratin 2e). The mab is reactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. It recognizes individual cells within epidermis of tongue and mamille (co-localization with keratin K1). It is also strongly reactive on hyperkeratosis of diverse origin.The mab does not react with palate keratin K76.

Reactivity: human, bovine, mouse

Applications: Immunohistochemistry on frozen and paraffin sections, Immunoblotting, Cytological material

Working DIlution: 1:200 for immunohistochemistry; 1:100 paraffin sections; 1:5,000 to 1:10,000 for immunoblotting (ECL method)

Storage: 2-8C

References:
- Collin, C. et al. (1992) Exp. Cell Res. 202: 132-241
- Swensson, O. et al. (1998) J. Dermatol. 139: 767-775