Cytokeratin/Keratin K76 (Keratin K2p) polyclonal, anti-human

Quantity: 25 µl

Background:
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13.

Synonyms:
KRT76, KRT2B, KRT2P, HUMCYT2A, K2P

Isotype: Guinea pig Polyclonal (K2p.1)

Immunogen:
Synthetic peptide (C-LGGAGSISVSHSGM) of human cy­tokeratin 2p, conjugated to KLH.

Form: Stabilized Serum

Specificity:
Basic human keratin K76 (Mr 64 000). Specific staining of keratin K76 in middle and upper suprabasal layers of the of palate and gingival epithelia. No cross-reaction with keratin K2! Negative with epithelia of foot sole, scalp, esophagus, duodenum, colon, vagina.

Reactivity: human

Applications: Immunohistochemistry on frozen and paraffin (after microwave treatment or trypsin) sections, Immunoblotting (western, ECL), Cytological material

Working DIlution: 1:200 for frozen sections; 1:100 for paraffin sections; 1:3000 for Immunoblotting

Storage: 2-8C

References:
- Collin C. et al. (1992) Differentiation 51: 137-148