Cytokeratin pan (Epithelial Marker) (clone AE-1/AE-3), anti-human, mouse, rat, monkey, cow, dog, rabbit, chicken
€0.00
In stock
SKU
ARP-24-MSM2-371
Catalog Number: 24-MSM2-371
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG's
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG's
Decription:
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Synonyms: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39
Clone: AE-1/AE-3
Isotype: Mouse IgG's
Immunogen: Human epidermal keratin
Reactivity: human, monkey, cow, dog, rabbit, mouse, rat, chicken. Others not known.
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blotting (0.5-1ug/ml for 2 hours at RT)
Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Reactivity: Skin, Adeno- or Squamous carcinomas
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Synonyms: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39
Clone: AE-1/AE-3
Isotype: Mouse IgG's
Immunogen: Human epidermal keratin
Reactivity: human, monkey, cow, dog, rabbit, mouse, rat, chicken. Others not known.
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blotting (0.5-1ug/ml for 2 hours at RT)
Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Reactivity: Skin, Adeno- or Squamous carcinomas
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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