DAP12 polyclonal, anti-human

Background:
Natural killer (NK) cells are regulated by stimulatory and inhibitory signals from a variety of receptors. Three main receptor families are responsible for NK cells recognition of MHC I molecules, including Ly-49, CD94/NKG2 and KIR (killer-cell inhibitory receptor). DAP12 is a phosphoprotein that is involved in the activation of NK cells. This protein interacts with membrane glycoproteins of the KIR family, resulting in cellular activation. DAP12 also binds to CD94/NKG2C, an activating NK cell receptor belonging to the C-type lectin superfamily. Additional proteins that bind to DAP12 include Ly-49D and Ly-49H, which associate with DAP12 in the plasma membrane. Phosphorylated DAP12 binds to ZAP-70 and Syk, suggesting that the activation pathway may be similar to that of the T and B cell antigen receptors.

Alternative Name:
KAR-associated protein, TYROBP, DAP12, KARAP, DAP 12, DAP12, DNAX activation protein 12, DNAX-activation protein 12, KAR-associated protein, KARAP, Killer activating receptor associated protein, Killer-activating receptor-associated protein, PLOSL, TYOBP, TYRO protein tyrosine kinase binding protein, TYRO protein tyrosine kinase-binding protein, TYROBP,

Application Dilution:
WB:1:500-1:1,000
IHC:1:50-1:200
FC:1:50-1:100

Specificity: DAP12 polyclonal antibody detects endogenous levels of DAP12 protein.

Immunogen:
Synthetic peptide within human DAP12 aa 60-100.

MW: 12 kDa

Swiss-Prot: O43914(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.