DARPP32 polyclonal, anti-human, mouse, rat

Background:
Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson's disease and drug abuse. DARPP-32 (for dopamine and adenosine 3',5'-monophosphate-regulated phosphoprotein) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1.

Alternative Name:
PPP1R1B, DARPP 32, DARPP-32, Dopamine and cAMP regulated neuronal phosphoprotein 32, Dopamine and cAMP regulated neuronal phosphoprotein, Dopamine and cAMP regulated phosphoprotein, Dopamine and cAMP regulated phosphoprotein DARPP 32, Dopamine and cAMP regulated phosphoprotein DARPP32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, IPPD, Neuronal phosphoprotein DARPP 32, PPP1R1B, PPR1B, Protein phosphatase 1 regulatory (inhibitor) subunit 1B, Protein phosphatase 1 regulatory inhibitor subunit 1B, Protein phosphatase 1 regulatory subunit 1B,

Application Dilution:
WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: DARPP32 polyclonal antibody detects endogenous levels of DARPP32 protein.

Immunogen:
recombinant protein

MW: 32 kDa

Swiss-Prot: Q9UD71(Human) Q60829(Mouse) Q6J4I0(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.