DCTN1 polyclonal, anti-human, mouse, rat

Background:
This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Synonyms: P135, DP-150, DAP-150

Cellular Location: Cytoplasm

Immunogen:
Recombinant protein of human DCTN1

Gene Symbol: DCTN1

Gene ID: 1639

Swiss prot: Q14203

Calculated MW: 142kDa

Recommended dilution:
WB 1:200-1000, IHC 1:20-100,

Purity:
Affinity purification

Storage Buffer:
Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.

Storage:
Store at -20℃. Avoid freeze / thaw cycles.