DDIT3 / CHOP Polyclonal Antibody

Background:
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Research Area: Cancer, Epigenetics and Nuclear Signaling, Metabolism, Developmental Biology, Stem Cells

Synonyms:
DDIT3, CEBPZ, CHOP, CHOP-10, CHOP10, GADD153, C/EBPzeta, DDIT3 / CHOP

Immunogen: A synthetic peptide of human DDIT3 / CHOP (NP_004074.2).

Swissprot: P35638
Gene ID: 1649

Calculated MW: 19 kDa/21 kDa
Observed MW: 25 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.