DDX4/MVH polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
DDX4(DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al.(2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog(Ddx4) resides(Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al.(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.

Description:
Rabbit IgG polyclonal antibody for Probable ATP-dependent RNA helicase DDX4 (DDX4) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.

Synonyms:
DDX 4 antibody, Ddx4 antibody, DDX4_HUMAN antibody, DEAD(Asp Glu Ala Asp) box polypeptide 4 antibody, DEAD box protein 4 antibody, Dead-box protein 4 antibody, DEAD/H(Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody, MGC111074 antibody, MVH antibody, Probable ATP dependent RNA helicase DDX4 antibody, Probable ATP-dependent RNA helicase DDX4 antibody, VASA antibody, VASA homolog antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human DDX4(253-272aa EDEDSIFAHYQTGINFDKYD), identical to the the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P, IHC-F, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.