Dehydroepiandrosterone (DHEA) test kit

Use: Used to detect the concentration of dehydroepiandrosterone (DHEA) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled dehydroepiandrosterone (DHEA) is used, and purified anti-dehydroepiandrosterone (DHEA) antibodies are coated on microplates. In the competitive inhibition reaction, a certain amount of solid-phase antibodies and biotin-labeled dehydroepiandrosterone (DHEA) and non-labeled antigens (calibrators or specimens) inhibit the competitive reaction. The binding amount of the antibody and biotin-labeled dehydroepiandrosterone (DHEA) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less the antibody and biotin-labeled dehydroepiandrosterone (DHEA) bind, and vice versa. After the reaction is balanced, a solid-phase antibody-biotinylated dehydroepiandrosterone (DHEA) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated dehydroepiandrosterone (DHEA)-enzyme label-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of dehydroepiandrosterone (DHEA) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation, and rapidity, and has reliable detection performance for the decrease or increase of dehydroepiandrosterone (DHEA) in the sample.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.