Desmoglein 2 polyclonal, anti-human

Quantity: 100 µl

Background:
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. This gene product is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10.

Synonyms:
HDGC, CDHF5, ARVC10, ARVD10, CMD1BB

Isotype: Rabbit Polyclonal

Immunogen:
Recombinant peptide (extracellular repeat domain E2 of human Desmoglein 2)

Form: Stabilized Serum

Specificity:
Antibody recognizes desmoglein 2 present in all epithelia and in tumors derived there from as in heart muscle and dendritic reticulum ells of lymphatic follicles. In basal epithelial cells demogelin 2 expression is generally very low. Reactivities on cultured cell lines: Many, including CaCo-2, HaCaT, MCF-7.

Reactivity: human

Applications: Western blot technique, Immunohistochemistry on cryostat sections, ELISA

Working DIlution: 1:20 for Immunohistochemistry; 1:600 for Western blotting (ECL)

Positive Control:
CaCo-2, HaCaT

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
- Schafer, S. et al. (1996) Differentiation 60: 99-108
- Pieperhoff S & Franke WW (2007) Eur J Cell Biol 86: 377-391