DHFR Polyclonal Antibody

Background:
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene.

Research Area: Cancer, Cell Biology, Metabolism, Signal transduction

Synonyms:
DHFR, DHFRP1, Dihydrofolate reductase, DYR, DYR, EC 1.5.1.3

Immunogen: Fusion protein of human DHFR

Swissprot: P00374
Gene Accession: BC000192

Calculated MW: 21 kDa
Observed MW: Refer to figures

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 0.96 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:50-1:300, ELISA 1:5000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.