DIAPH1 polyclonal, anti-human

Background:
Dia 1, also known as DIAPH1 (diaphanous homolog 1) or DRF1, a mammalian homolog of the Drosophila diaphanous gene, belongs to a family of formin homology (FH) proteins which are characterized by having tandemly aligned FH1 (formin homology 1) and FH2 (formin homology 2) domains in their carboxy terminal regions. Dia 1 contains a DAD (diaphanous autoregulatory) domain, which is involved in the elongation of actin filaments, and a GBD/FH3 (Rho GTPase-binding/formin homology 3) domain, which interacts with the DAD domain via autoinhibitory interactions to regulate the activation of Dia 1. Dia 1 is required for the assembly of F-actin structures, and regulates the polymerization and depolymerization of actin filaments. Localizing to the cell membrane, Dia 1 is expressed in a wide range of tissues, including brain, heart, lung and kidney. Defects to the gene encoding Dia 1 have been linked to deafness autosomal dominant type 1 (DFNA1), a disorder characterized by sensorineural hearing loss..

Alternative Name:
DRF1, DIAPH1, DIAP1, DIAPH1, deafness, autosomal dominant 1, DFNA1, DIA1, DIAP1, DIAP1, DIAPH1, Diaphanous homolog 1 (Drosophila), diaphanous homolog 1, Diaphanous related formin 1, Diaphanous-related formin-1, DRF1, FLJ25265, hDIA1, LFHL1, low frequency hearing loss 1, p140DIA, Protein diaphanous homolog 1,

Application Dilution:
WB:1:5,000-1:10,000
ICC:1:50
IHC:1:50-1:100

Specificity: DIAPH1 polyclonal antibody detects endogenous levels of DIAPH1 protein.

Immunogen:
Recombinant protein corresponding to N-terminal human DIAPH1.

MW: 141 kDa

Swiss-Prot: O60610(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.