DIAPH2 polyclonal, anti-human, mouse, rat
€305.00
In stock
SKU
BS60413
Background:
Dia 1 (also designated p140mDia) and Dia 2 (also designated p134mDia 2), mammalian homologs of the Drosophila diaphanous gene, belong to a family of formin homology (FH) proteins which are characterized by having tandemly aligned FH1 and FH2 domains in their carboxy terminal regions. The amino terminus of inactivated Dia 1 binds to the FH1 and FH2 domains and is activated when GTP-bound Rho disrupts the intramolecular interaction of Dia 1 and converts it to an open conformation. Once activated, Dia 1 co-localizes with Profilin and RhoA in dynamic plasma membrane structures and induces the formation of stress fibers in conjunction with the ROCK-mediated signaling pathway. However, Dia 1 and ROCK activities antagonize each other in order to determine the thickness and density of the stress fibers formed.
Alternative Name:
Protein diaphanous homolog 2, Diaphanous-related formin-2, DRF2, DIA, Diap2, Dia2, Dia 2
Application Dilution: WB: 1:500~1:1000
Specificity: DIAPH2 polyclonal antibody detects endogenous levels of DIAPH2 protein.
Immunogen:
A synthetic peptide corresponding to residues in Human DIAPH2.
MW: ~ 130 kDa
Swis Prot.: O60879
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Dia 1 (also designated p140mDia) and Dia 2 (also designated p134mDia 2), mammalian homologs of the Drosophila diaphanous gene, belong to a family of formin homology (FH) proteins which are characterized by having tandemly aligned FH1 and FH2 domains in their carboxy terminal regions. The amino terminus of inactivated Dia 1 binds to the FH1 and FH2 domains and is activated when GTP-bound Rho disrupts the intramolecular interaction of Dia 1 and converts it to an open conformation. Once activated, Dia 1 co-localizes with Profilin and RhoA in dynamic plasma membrane structures and induces the formation of stress fibers in conjunction with the ROCK-mediated signaling pathway. However, Dia 1 and ROCK activities antagonize each other in order to determine the thickness and density of the stress fibers formed.
Alternative Name:
Protein diaphanous homolog 2, Diaphanous-related formin-2, DRF2, DIA, Diap2, Dia2, Dia 2
Application Dilution: WB: 1:500~1:1000
Specificity: DIAPH2 polyclonal antibody detects endogenous levels of DIAPH2 protein.
Immunogen:
A synthetic peptide corresponding to residues in Human DIAPH2.
MW: ~ 130 kDa
Swis Prot.: O60879
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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