DLK1 (clone PF299-1), anti-human

Decription:
DLK1 (Protein delta homolog 1; Preadipocyte factor 1; Pref-1) is a regulator of adipocyte differentiation found in serum and urine. DLK1 might be a decoy ligand. Serum or plasma levels of soluble DLK1 affect negatively or positively adipogenesis and control mesenchymal cell fate. DLK1 is frequently upregulated in myelodysplastic syndrome (MDS) patients compared to non-leukemic individuals.

Synonyms: Protein Delta Homolog 1, Fetal Antigen 1, Preadipocyte Factor 1, Pref-1, FA1

Clone: PF299-1

Isotype: Mouse IgG1 kappa

Immunogen: Recombinant human DLK1 (extracellular domain).

Reactivity: human

Specificity: Recognizes human DLK1. Does not cross-react with mouse DLK1.

Form: Purified, in 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Applications:
ELISA: (direct or indirect: 1:2'000-1:10'000)
Flow Cytometry: (1:100-1:500)
Immunohistochemistry: (paraffin sections (1:100-1:500))
Western blot: (1:2'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).

Working Dilution: Optimal conditions must be determined individually for each application.

Concentration: 1mg/ml

Storage: 2-8C immediate use, or -20C (aliquote)