DNA Ligase I polyclonal, anti-human, mouse
€388.00
In stock
SKU
BS6652
Background:
Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I. A combination of DNA polymerase epsilon, PCNA, replication factor C, replication protein A, and DNA ligase I is well-suited to the task of creating nucleotide excision repair patches.
Alternative Name:
DNA ligase 1, DNA ligase I, DNLI1, LIG 1, lig1, Ligase I DNA ATP dependent, Polydeoxyribonucleotide synthase [ATP] 1,
Application Dilution: WB: 1:500~1:2000, IHC/IF: 1:50~1:200
Specificity: DNA Ligase I polyclonal antibody detects endogenous levels of DNA Ligase I protein.
Immunogen:
Recombinant full length Human DNA Ligase I.
MW: ~ 102 kDa
Swis Prot.: P18858
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I. A combination of DNA polymerase epsilon, PCNA, replication factor C, replication protein A, and DNA ligase I is well-suited to the task of creating nucleotide excision repair patches.
Alternative Name:
DNA ligase 1, DNA ligase I, DNLI1, LIG 1, lig1, Ligase I DNA ATP dependent, Polydeoxyribonucleotide synthase [ATP] 1,
Application Dilution: WB: 1:500~1:2000, IHC/IF: 1:50~1:200
Specificity: DNA Ligase I polyclonal antibody detects endogenous levels of DNA Ligase I protein.
Immunogen:
Recombinant full length Human DNA Ligase I.
MW: ~ 102 kDa
Swis Prot.: P18858
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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