DNASE1 polyclonal, anti-human, mouse
€388.00
In stock
SKU
BS7729
Background:
Internucleosomal DNA fragmentation following the activation of endonucleases is the common end point of apoptosis. DNase I, a Ca2+/Mg2+-dependent endonuclease ubiquitously expressed in mammalian tissues, has been implicated to mediate internucleosomal DNA degradation in human cells undergoing apoptosis. DNase I is highly polymorphic, and at least six alleles of DNase I are known. DNase II, the ubiquitously expressed acidic deoxyribonuclease, acts downstream of caspase activation and may also induce DNA digestion during apoptosis. DNase I cleaves DNA to 5'-phosphodinucleotide and 5'-phospho- oligonucleotide end-products, whereas DNase II cleaves DNA to 3'-phosphomononucleotide and 3'-phosphooligonucleotide end-products. The mechanism by which DNase II cuts DNA is similar to DNase I, which produces nicks rather than double-strand cuts. DNase II is usually present in cytoplasm of epithelial cells, but it appears concentrated in the nuclei of lens fibers. In contrast, DNase I is always concentrated in nuclei of epithelial and fiber cells.
Alternative Name:
Deoxyribonuclease 1, Deoxyribonuclease I, Deoxyribonuclease-1, Deoxyribonuclease1, DeoxyribonucleaseI, DNAS1_HUMAN, DNASE 1, DNase I, DNase I lysosomal, DNASE1, DNaseI, DNL 1, DNL1, Dornase alfa, DRNI, FLJ38093, Human urine deoxyribonuclease I,
Application Dilution: WB: 1:500~1:2000, IF: 1:50~1:200
Specificity: DNASE1 polyclonal antibody detects endogenous levels of DNASE1 protein.
Immunogen:
Recombinant full length Human DNASE1.
MW: ~ 31 kDa
Swis Prot.: P24855
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Internucleosomal DNA fragmentation following the activation of endonucleases is the common end point of apoptosis. DNase I, a Ca2+/Mg2+-dependent endonuclease ubiquitously expressed in mammalian tissues, has been implicated to mediate internucleosomal DNA degradation in human cells undergoing apoptosis. DNase I is highly polymorphic, and at least six alleles of DNase I are known. DNase II, the ubiquitously expressed acidic deoxyribonuclease, acts downstream of caspase activation and may also induce DNA digestion during apoptosis. DNase I cleaves DNA to 5'-phosphodinucleotide and 5'-phospho- oligonucleotide end-products, whereas DNase II cleaves DNA to 3'-phosphomononucleotide and 3'-phosphooligonucleotide end-products. The mechanism by which DNase II cuts DNA is similar to DNase I, which produces nicks rather than double-strand cuts. DNase II is usually present in cytoplasm of epithelial cells, but it appears concentrated in the nuclei of lens fibers. In contrast, DNase I is always concentrated in nuclei of epithelial and fiber cells.
Alternative Name:
Deoxyribonuclease 1, Deoxyribonuclease I, Deoxyribonuclease-1, Deoxyribonuclease1, DeoxyribonucleaseI, DNAS1_HUMAN, DNASE 1, DNase I, DNase I lysosomal, DNASE1, DNaseI, DNL 1, DNL1, Dornase alfa, DRNI, FLJ38093, Human urine deoxyribonuclease I,
Application Dilution: WB: 1:500~1:2000, IF: 1:50~1:200
Specificity: DNASE1 polyclonal antibody detects endogenous levels of DNASE1 protein.
Immunogen:
Recombinant full length Human DNASE1.
MW: ~ 31 kDa
Swis Prot.: P24855
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
| Is Featured? | No |
|---|
Write Your Own Review