Dnmt1 polyclonal, anti-human, mouse, rat

Background:
Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.

Alternative Name:
Dnmt1, DNMT1, ADCADN, AIM, CXXC finger protein 9, CXXC-type zinc finger protein 9, CXXC9, DNA (cytosine 5 ) methyltransferase 1, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase 1, DNA methyltransferase HsaI, DNA methyltransferase M.HsaI., DNA MTase, DNA MTase HsaI, DNMT 1, DNMT, Dnmt1, DNMT1, Dnmt1o, FLJ16293, HSN1E, M.HsaI, MCMT, Met1, MGC104992, mMmul, MommeD2,

Application Dilution:
WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Specificity: Dnmt1 polyclonal antibody detects endogenous levels of Dnmt1 protein.

Immunogen:
recombinant protein

MW: 183 kDa

Swiss-Prot: P26358(Human) P13864(Mouse) Q9Z330(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.