Dnmt3a monoclonal, anti-human, mouse, rat

Description: Anti-Dnmt3a Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Protein Function:
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity..

Other Names: DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, 2.1.1.37, Cysteine methyltransferase DNMT3A, 2.1.1.-, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA, DNMT3A

Clone Name(s):
Clone: EOI-4

Subcellular Localization: Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin..

Tissue Specificity: Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung..

Gene ID: 1788

Immunogen: A synthesized peptide derived from human Dnmt3a

Calculated MW: 101858

Purification: Affinity-chromatography

Format: Liquid

Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Concentration (mg/ml): Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.