Dog F8 (Coagulation Factor VIII) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor VIII(F8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor VIII(F8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor VIII(F8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor VIII(F8) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Metabolic pathway, Hematology

Alternative Names:
FVIII, AHF, HEMA, F8B, F8C, Antihemophilic Globulin, Hemophilia A, Anti-Hemophilic Factor A, FVIII Procoagulant Component, F8 Protein

Sample Type:
plasma

Assay Type: Sandwich

Standard: 5000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only