Dog MIP3b (Macrophage Inflammatory Protein 3 Beta) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 3 Beta(MIP3b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 3 Beta(MIP3b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 3 Beta(MIP3b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 3 Beta(MIP3b) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cytokine, Infection immunity

Alternative Names:
CCL19, CKb11, ELC, MIP3-B, SCYA19, Chemokine C-C-Motif Ligand 19, Beta Chemokine Exodus-3, CK Beta-11, EBI1-Ligand Chemokine, Small Inducible Cytokine Subfamily A19

Sample Type:
Serum, plasma and other biological fluids.

Assay Type: Sandwich

Standard: 1000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only