Dog serum proteins polyclonal FITC, anti-canine

Quantity: 200 µl

Background:
Tested in immunoelectrophoresis and double radial immunodiffusion against pooled serum precipitation of at least 12 individual proteins have been observed. Direct fluorescent staining of dog serum proteins at the cellular and subcellular level of appropriately treated cell and tissue substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Isotype: Rabbit Polyclonal

Source: Pooled whole serum and selected protein fractions isolated from pooled dog serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: FITC-coupled purified hyperimmune rabbit IgG, in phosphate buffered saline (PBS, pH 7.2).

Reactivity: Canine

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence

Storage: Store at 4C, or in small aliquots at -20C.