Drebrin polyclonal, anti-human, bovine, rat, mouse

Quantity: 25 µl

Background:
Drebrin is a protein that in humans is encoded by the DBN1 gene. The protein encoded by this gene is a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain.

Synonyms:
DBN, DBN1

Isotype: Guinea pig Polyclonal (gp drebE2/A)

Immunogen:
Synthetic human peptide (aa 324-343) conjugated to KLH

Form: Stabilized Serum

Specificity:
The antiserum specifically reacts with drebrin isoforms E2 and A, widespread actin-associating proteins of 70 kD (SDS-PAGE mobility shows Mr 120,000). Drebrin has originally been described only in neuronal cells, but has been recently found also in the actin-rich lamellipodia and filopodia of motile cells. It is enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells. Positive staining was found in many tissues, including diverse epithelia and carcinoma (e.g. in epidermis basal cells are positive, whereas overlying cells are negative; also positive: regenerating epithelium during wound healing; basal cell carcinoma; skin melanoma), specific types of endothelia and smooth muscle; especially prominent in the mesangial cells of renal glomeruli and in the Sertoli cells of testis. Also positive: epithelia of hair follicles and eccrine sweat glands. Consistently negative were, however, hepatocytes and cross-striated muscle.

Reactivity: human, bovine, rat, mouse

Applications: Immunohistochemistry on frozen (paraformaldehyde fixed) and paraffin (microwave treatment) sections; Immunofluorescence microscopy; Immunoblotting (western, ECL); Immunoprecipitation; Cytological material

Working DIlution: 1:100 for immunohistochemical applications

Positive Control:
Reactivitieson Cultured Cell Lines: PLC, U333, HUVEC, HaCat, HeLa, MCF-7, SV-80; B1; B16 (mouse melanoma cells)

Storage: 2-8C

References:
- Peitsch WK. et al. (1999) Eur J Cell Biol 78: 767-778
- Peitsch WK. (2001) Eur J Cell Biol 80: 567-579
- Peitsch WK. et al. (2005) J Invest Dermatol 125: 761-774