DROSHA Polyclonal Antibody

Background:
This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer.

Research Area: Cancer, Epigenetics and Nuclear Signaling

Synonyms:
DROSHA, ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN

Immunogen: A synthetic peptide of human DROSHA (NP_037367.3).

Swissprot: Q9NRR4
Gene ID: 29102

Calculated MW: 138 kDa/151 kDa/155 kDa/159 kDa
Observed MW: 180 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000 IF 1:50-1:200"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.