DUT polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Deoxyuridine triphosphate nucleotidohydrolase(dUTPase) is responsible for maintaining low intracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA. dUTPase activity/expression can be down-regulated using siRNA specifically targeted to dUTPase mRNA and dUTPase plays a role in DNA nucleotide metabolism. This protein, present predominantly in the cytoplasm, contains 252 amino acids with a Mr of 26,704. It exhibits 35% identity with the E.coli dUTPase and 53% identity with the Saccharomyces cerevisiae enzyme. The nuclear and mitochondrial forms of dUTPase are encoded by the same gene with isoform-specific transcripts arising through the use of alternative 5-prime exons. Human dUTPase exhibits 92% identity with rat. Moreover, this enzyme has profound effects on the efficacy of agents that target thymidylate biosynthesis.

Description:
Rabbit IgG polyclonal antibody for Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial (DUT) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody, Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody, Deoxyuridine triphosphatase antibody, dut antibody, DUT_HUMAN antibody, dUTP nucleotidohydrolase antibody, dUTP pyrophosphatase antibody, dUTPase antibody, FLJ20622 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human DUT(212-229aa KKGDRIAQLICERIFYPE), different from the related rat and mouse sequences by two amino acids.A synthetic peptide corresponding to a sequence at the C-terminus of human DUT(212-229aa KKGDRIAQLICERIFYPE), different from the related rat and mouse sequences by two amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.