DUT Polyclonal Antibody

Background:
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

Research Area: Epigenetics and Nuclear Signaling

Synonyms:
Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform, Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial, Deoxyuridine triphosphatase, dut, DUT, dUTP nucleotidohydrolase, dUTP pyrophosphatase, dUTPase, FLJ20622

Immunogen: Fusion protein of human DUT

Swissprot: P33316
Gene Accession: BC033645

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 1.08 mg/mL
Dilution: IHC 1:50-1:300, ELISA 1:5000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.